Fluoride Inhibition of [2- 14c]thymidine Incorporation into Dna in Mung Bean Seedlings

نویسندگان

  • A Narita
  • Y Nakamura
  • A Shigematsu
چکیده

The effect of sodium fluoride (NaF) on the incorporation of [2-14C] thymidine into DNA in germinating mung bean (Vigna radiate cv Uthong-1) seedlings was studied. One-day-old mung bean seedlings were treated with water (control) or 1.0 mM NaF containing [2-14C]thymidine for 24 h and the thymidine incorporation into various parts of the radicle was examined. Silver grains shown by the autoradiograph of the F-exposed radicle were markedly decreased compared with those of the control. Fission rates (%) exhibited by various parts of the experimental radicles were much lower than those of the control. The results indicated that NaF suppressed DNA synthesis in mung bean seedlings.

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تاریخ انتشار 2009